Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells

Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin.

Strains of Bacteroides fragilis associated with diarrheal disease (enterotoxigenic B. fragilis) produce a 20-kDa zinc-dependent metalloprotease toxin (B. fragilis enterotoxin; BFT) that reversibly stimulates chloride secretion and alters tight junctional function in polarized intestinal epithelial cells. BFT alters cellular morphology and physiology most potently and rapidly when placed on the ...

Detection of Bacteroides fragilis enterotoxin gene by PCR.

Bacteroides fragilis constitutes about 1% of the bacterial flora in intestines of normal humans. Enterotoxigenic strains of B. fragilis have been associated with diarrheal diseases in humans and animals. The enterotoxin produced by these isolates induces fluid changes in ligated intestinal loops and an in vitro cytotoxic response in HT-29 cells. We developed a nested PCR to detect the enterotox...

7-ketocholesterol induces endoplasmic reticulum stress in HT-29 cells.

7-Ketocholesterol (7-Kchol, oxidized cholesterol) is an important mediator of cell death in atherosclerosis mediated by up-regulated Nox 4 gene expression. In the current study using the human colon cancer HT-29 cell line, we have demonstrated that 7-Kchol promotes endoplasmic reticulum (ER) stress via gene up-regulation of ER chaperone and membrane kinases.

Heterogeneity in responses by primary adult human colonic epithelial cells to purified enterotoxin of Bacteroides fragilis.

BACKGROUND Enterotoxigenic strains of Bacteroides fragilis (ETBF) have been implicated in diarrhoeal illness in livestock and children, but their role in adult human colonic disease is unknown. AIMS To investigate responses by primary adult human colonic epithelial cells to purified B fragilis toxin (BFT). METHODS BFT was purified from culture supernatant of a highly toxigenic strain of ETB...

Endothelial Cells via an Aldose Reductase-, Intercellular Adhesion Molecule-1 in Enterotoxin Upregulates Bacteroides fragilis